![]() On November 24, 2021, the World Health Organization reported the occurrence of omicron variant in South African countries and on Novemit was on the list of variants of concern. Omicron was discovered in early November, 2021 in Botswana. The World Health Organization has designated VOCs as alpha, beta, delta, and gamma, containing mutations having a certain set of characteristics. Since the start of the pandemic, several variants were discovered along with the variants of concern (VOCs) that have major mutations with increased transmissibility, severity of the disease and resistance to vaccine 1. The current study identified a Multi-Epitope Vaccine (MEV) as a significant vaccine candidate that could potentially help the global world to combat SARS-CoV-2 infections. Several computational tools were used to predict and analyze the vaccine constructed by using spike protein sequence of omicrons. coli using the SnapGene tool for the expression study and a good immune response was observed. The constructed vaccine was ligated in pET-28a (+) vector in E. Subsequently, vaccine structure was docked with the receptor and cloned in a pET-28a (+) vector. The epitopes were linked with 3 types of linker EAAAK, AAY and GPGPG for vaccine construction. Multiple epitopes were selected on the basis of toxicity, immunogenicity and antigenicity, and vaccine subunit was constructed having potential immunogenic properties. Through this approach, novel epitopes of the S protein-SARS-CoV-2 were predicted for the development of a multiple epitope vaccine. The aim of the present study is to develop a particular vaccine exploiting bioinformatics approaches which can target B- and T-cells epitopes. To avoid the risk of arising viral illness, the construction of a specific vaccine that triggers the production of targeted antibodies to combat infection remains highly imperative. The susceptibility to omicron variant by immunization-induced antibodies are direly required for risk evaluation. ProtCID - Protein common interface database (for homologous proteins).The World Health Organization categorized SARS-CoV-2 as a variant of concern, having numerous mutations in spike protein, which have been found to evade the effect of antibodies stimulated by the COVID-19 vaccine.Proteopedia: essentially a wikipedia for proteins. ![]()
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